Application Results

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fig 6: Graph of number of infected lettuce against number of days

 

Graph 6 shows the number of infected lettuce over a period of 14 days. At the start, both tests had more infected lettuce pieces than the control. However, towards the end of 14 days, control showed more pieces of lettuce infected with bacterial soft rot, followed by tannic acid and then tea. 

 

 

 

 

 

 

 

 

 

 

 

 

 

                      Fig7a: Lettuce starting to      Fig 7b:  Lettuce turning         Fig 7c: Able to see through the      Fig 7d: Veins can be clearly

                                rot                                 brown in colour                    leaf                                                  seen. Leaf breaking up into                                                                                                                                                                                                            pieces

As seen from Figure 7a to d, the process of soft rot begins with the browning of the surface of the lettuce. As the bacteria continue to produce increasing amounts of pectolytic enzymes within the lettuce which further break down tissues, the lettuce becomes more translucent. Towards the end of the rotting process, veins in the lettuce can be clearly seen, and the lettuce becomes softened to the extent of breaking apart.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Graph 2: Graph of lettuce results

 

At the end of 14 days, the percentage area of lettuce covered with soft rot was measured. Control had an average of 64.49%, tannic acid had 46.59%, and green tea had 41.46%. Although through the one-way anova test, there was an insignificant difference between the tests, we could still see an observable difference between the tests and control.

P value = 0.208 > 0.05

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                Fig 8: Lettuce piece showing signs of soft rot         Fig 8a: Lettuce piece not showing signs of soft rot